. Author manuscript; available in PMC: 2012 Mar 1.

Published in final edited form as: Glia. 2010 Dec 29;59(3):472–485. doi: 10.1002/glia.21117

Table 2.

Quantification of Glioma Infiltrating Microglia/Macrophages (GIMs) density, proliferation and apoptosis

Tumor treatment	Saline	tuftsin	MIF/TKP
Measurement	Saline	tuftsin	MIF/TKP
GIM density (lectin⁺ cells/mm²)	687±23.9	1014±176.8^*	495±51.7^**

Total proliferation (Ki67⁺ cells/mm²)	2354.7±225.0	2839.5±164.0^*	1386.0±219.7^**
Tumor cell proliferation (Ki67⁺GFP⁺ cells/mm²)	1848.1±130.3	2207.6±78.5^**	1322.7±267.9^*

Total apoptosis (activated caspase 3⁺/mm²)	48.6±4.6	28.0±0.9^**	60.4±5.2^*
Tumor cell apoptosis (activated caspase 3⁺GFP⁺ clls/mm²)	20.9±3.8	13.4±0.8^*	15.2±2.1
GIM apoptosis (activated caspase 3⁺lectin⁺ cells/mm²)	4.2±0.5	2.8±0.5^*	17.3±4.1^**

GIM density was measured by counting MG/MP number per field inside tumor area. The proliferation and apoptosis of tumor tissue, tumor cells and GIM in saline group, D0 tuftsin group and D5 MIF/TKP group were quantified by Ki67 and activated caspase-3 immunofluorescent staining (n = 3)

Data are presented as mean±SEM. and

^**

indicate the statistical analysis between the experimental group and saline control group.