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. Author manuscript; available in PMC: 2012 Mar 4.
Published in final edited form as: Circ Res. 2011 Jan 20;108(5):544–554. doi: 10.1161/CIRCRESAHA.110.231803

Figure 4. 15-LO mRNA expression is inhibited by αM integrin activation.

Figure 4

Human monocytes were incubated in the presence of activating antibody to β2 integrin or clustering through αM integrin (as described in the Methods section) followed by stimulation with IL-13 for 24 hours. After incubation monocyte mRNA was isolated using a Qiagen Oligotex mRNA Midi Kit and levels of 15-LO mRNA were evaluated by quantitative real time RT-PCR. After normalization with GAPDH amplification, the fold induction of 15-LO mRNA expression after β2 integrin activation or αM integrin clustering was compared with the control. Data were collected from five independent experiments and are shown as means ± SE. Statistical analysis was performed using Student’s paired t-test, (* P< 0.05 compared to IL-13 stimulated cells).