Figure 2.

C1q modifies cholesterol distribution and decreases cholesterol levels in neurons. A, C1q alters cholesterol distribution in neurons. Neurons cultured with or without C1q for 24 h were stained with filipin and anti MAP-2 antibodies and analyzed by fluorescence microscopy. Scale bars, 10 μm. B, Quantification of cholesterol content in untreated (UT) and C1q-treated neurons by enzymatic fluorometric assay over a 48 h time course. Results represent means ± SD (n = 3). Two-way ANOVA followed by Bonferroni's post hoc test, *p < 0.05 and **p < 0.01.