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. 2011 Mar 2;31(9):3459–3469. doi: 10.1523/JNEUROSCI.3932-10.2011

Figure 3.

Figure 3.

C1q increases stx3 expression and its interaction with SNAP25. A, B, C1q increases the protein levels of stx3 in neurons. Neurons cultured with or without C1q for 24 h were stained with anti-stx3. Nuclei are stained with DAPI. A, Representative micrographs of stx3 staining. B, Quantitative image analysis of stx3 expressed as means ± SD (n = 4, 7 fields per condition) of percentage of field area (□) or area ratio stx3/DAPI (■). Two-tailed nonparametric Mann–Whitney U test, *p < 0.05 and ***p < 0.001. C, Colocalization of stx3 and SNAP25 in C1q-treated neurons. Neurons cultured with or without C1q for 24 h were stained with anti-stx3 (Alexa Fluor 555, red) and anti-SNAP25 (Alexa Fluor 488, green) antibodies and analyzed by confocal microscopy. Representative micrographs of two independent experiments are shown with white boxed section expanded on the right. UT, Untreated. Scale bars, 10 μm. D, SNAP25 immunoprecipitates with stx3 after 24 h of stimulation with C1q. One blot representative of three independent experiments is shown. IP, Immunoprecipitation.