Figure 7.

C1q modulates mouse neuronal survival and gene expression. A, C1q promotes neurite outgrowth in primary cortical mouse neurons. Mouse neurons were incubated with or without C1q for 24 h, fixed, and stained with MAP-2 antibodies. Scale bars, 100 μm. B, Mouse and rat primary cortical neurons were incubated with or without C1q for 3 h, and gene expression was assessed by qRT-PCR. C, Mouse primary cortical neurons were incubated with 10 nm C1q, C1q tails, or heat-inactivated C1q (ΔHC1q) for 3 h, and gene expression was assessed by qRT-PCR. Results are expressed as the mean ± SD of log2 FC from triplicate samples from two independent experiments. UT, Untreated.