Figure 3. NG1427 is inducible following treatment with DNA damaging agents H₂O₂, and does not require RecA.

All data show relative expression of each target gene calculated using a control transcript omp3 for each condition tested. Error bars are +/− s.e.m, Asterisks denote p<0.05 as calculated by Student’s t-test. A: H₂O₂ treatment induces the NG1427 regulon in a recA-independent manner. Induction of the NG1427 regulon following treatment with 5 mM H₂O₂ was measured in recA6 Gc with or without IPTG induction. ng1427 transcript was measured with the “ng1427 probe” for each strain. B: The absence of RecA does not affect de-repression of the NG1427 regulon following H₂O₂ treatment. Induction of the NG1427 regulon following peroxide treatment was measured in parental, ng1427::cat, and recA4 Gc. ng1427 transcript was measured with the “ng1427 qPCR probe” for parental and recA4 strains, and the “ng1427::cat 5’ end qPCR probe” for the ng1427::cat strain. All data show a minimum of 3 biological replicates (n=3).