Skip to main content
. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 22;79(4):846–860. doi: 10.1111/j.1365-2958.2010.07491.x

Figure 4. NG1427 requires RecA for full regulon induction following treatment with DNA damaging agents MMC and MMS.

Figure 4

All data show relative expression of each target gene calculated using a control transcript omp3 for each condition tested. Error bars are +/− s.e.m, Asterisks denote p<0.05 as calculated by Student’s t-test. A: Full de-repression of the NG1427 regulon following MMS treatment requires expression of RecA. Induction of the NG1427 regulon after exposure to 0.02% (v/v) MMS was measured in parental, ng1427::cat, and recA4 Gc. ng1427 transcript was measured with the “ng1427::cat 5’ end qPCR probe” for all strains. B: Full de-repression of the NG1427 regulon following MMC treatment requires expression of RecA. Induction of the NG1427 regulon after exposure to 25 ng/ml conc. MMC was measured in parental, ng1427::cat and recA4 Gc. ng1427 transcript was measured with the “ng1427::cat 5’ end qPCR probe” for all strains. All data show a minimum of 3 biological replicates (n=3).