Figure 5. Treatment with ONOO− and NaOCl does not meaningfully affect the NG1427 regulon.

All data show relative expression of each target gene calculated using a control transcript omp3 for each condition tested. Error bars are +/− s.e.m, Asterisks denote p<0.05 as calculated by Student’s t-test. A: ONOO− treatment does not strongly induce de-repression of the NG1427 regulon. Induction of the NG1427 regulon following exposure to 3 mM conc. ONOO− was measured in parental, ng1427::cat and recA4 Gc. ng1427 transcript was measured with the “ng1427::cat 5’ end qPCR probe” for all strains. B: NaOCl treatment does not strongly induce de-repression of the NG1427 regulon. Induction of the NG1427 regulon following exposure to 1.7 mM conc. NaOCl was measured in parental, ng1427::cat and recA4 Gc. ng1427 transcript was measured with the “ng1427::cat 5’ end qPCR probe” for all strains. All data show a minimum of 3 biological replicates (n=3).