Figure 6. Disrupting cysteine 64 (Cys64) prevents RecA-mediated NG1427 autoproteolysis.

Western analysis of strain NG1427-C64S-HA following treatment with either H2O2,−ONOO−, NaOCl, MMC, or MMS. Treatment of NG1427-HA was done in parallel and is included as a positive control Arrow points to full-length protein, dotted arrow denotes NG1427-HA C-terminal cleavage product with HA tag.