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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 22;79(4):846–860. doi: 10.1111/j.1365-2958.2010.07491.x

Figure 7. NG1427 binds specifically to the putative promoter regions of the NG1427 regulon.

Figure 7

2 nM biotin-labeled DNA consisting of the ng1427/ng1428 intergenic region or the upstream recN intergenic region was incubated with 100 nM of NG1427-G113D and unlabelled competitor DNA in molar excess denoted. “EBNA,” Epstein-Barr nuclear antigen target DNA, is an irrelevant piece of DNA used as a negative control. Reactions were then separated by electrophoresis on a 5% acrylamide Tris-HCl gel. The solid arrow denotes NG1427 bound DNA. The dotted arrow denotes unbound DNA. The asterisk denotes a non-specific band found in all reactions.