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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Mol Microbiol. 2010 Dec 22;79(4):846–860. doi: 10.1111/j.1365-2958.2010.07491.x

Figure 8. NG1427 cannot bind DNA following oxidative treatment.

Figure 8

− 2 nM biotin-labeled DNA consisting of the ng1427/ng1428 intergenic region or the upstream recN intergenic region was incubated with 100nM of NG1427-G113D. Where indicated, NG1427-G113D was either left untreated or treated with a varying range of concentrations of A: H2O2, B: MMS, C: ONOO−, or D: NaOCl and incubated for 15 minutes following addition of DNA. The reaction was run on a 5% acrylamide Tris-HCl gel, transferred to a nylon membrane, and detected with streptavadin-HRP. The solid arrow denotes NG1427-G113D-bound DNA. The dotted arrow denotes unbound DNA. The asterisk denotes a non-specific band found in all reactions