Figure 1.

ChR2-Venus-expressing axons from TeA pass through EC and innervate LA. A, A representative slice containing TeA infected with ChR2-AAV. Left, Bright-field image. Middle, Fluorescence image (YFP) of the corresponding rectangle area; yellow dotted line indicates medial boundary of the external capsule. Right, Overlay of YFP-expressing areas at injection sites from 6 animals. B, A postrecording fixed LA slice under bright field illumination (left) and fluorescence (right, YFP). Recording was made from neurons within the yellow dotted triangle area. C, Confocal images of YFP-expressing fibers around EC from a mouse injected with ChR2-AAV in TeA. Magnified image represents yellow rectangular area containing LA EC.