FIG. 5.

PCB 77 induces AHR enhancer recruitment at a lower level but over a longer period of time. Hepa-1 cells were treated with 5μM BaP, 5nM TCDD, 5000nM PCB77, or vehicle. At 30-min intervals, cells were fixed, and ChIP was performed using AHR-specific antibodies. QRT-PCR with specific primers was performed to determine the amount AHR recruited to the enhancer. PCR was done in duplicate, and the experiment was repeated twice. Each sample’s percent total input was set relative to vehicle control and plotted as a function of time. Error bars represent SEs. An * denotes statistical significance when compared with the TCDD at the same time point (p value < 0.05).