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. 2011 Apr 18;193(2):347–363. doi: 10.1083/jcb.201010034

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© 2011 Day et al.

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Figure 4. — Disruption of AKAP binding removed the punctate pattern. (A) Control GFP empty vector, GFP-tagged wild-type (wt) RIα, and GFP-tagged deletion mutants, RIα(1–93) and RIα(93–381), were expressed in Prkar1a^−/− MEFs. White arrows indicate a few of the faint puncta that were seen with the expression of RIα(1–93)-GFP. (B) A mutation that has been reported to disrupt D-AKAP1 binding, RIα(I27A), was GFP-tagged and expressed in Prkar1a^−/− MEFs. GFP empty vector and GFP-tagged wild-type RIα were included as controls. (C) mCherry empty vector or mCherry-tagged AKB domain from D-AKAP2 (RI/RII selective, RI selective, or RII selective) was coexpressed with GFP-tagged RIα in Prkar1a^−/− MEFs. Bars, 20 µm.