Table II.
Strains | Time of MEN | Time of final breakdown | Final spindle length | n |
WT | 5.8 (4.2) | 28.5 (4.5) | 11.00 (0.95) | 15 |
pGAL1-SLI15 | 36.3 (11.0) | 43.3 (12.7) | 9.51 (1.32) | 20 |
pGAL1-CTF13 | 17.4 (6.1) | 32.9 (5.6) | 8.73 (0.51) | 11 |
bir1Δ591–640 | 38.9 (21.6) | 56.6 (20.4) | 8.64 (0.97) | 8 |
bub2Δ | 9.1 (4.7) | 29.5 (5.5) | 9.87 (1.60) | 8 |
bub2Δ pGAL1-SLI15 | 8.9 (6.4) | 31.0 (5.5) | 10.01 (1.67) | 8 |
bub2Δ pGAL1-CTF13 | 4.2 (6.4) | 25.0 (3.4) | 8.75 (1.16) | 9 |
bub2Δ bir1Δ591–640 | 1.3 (9.0) | 33.5 (7.7) | 9.48 (2.82) | 5 |
cin8Δ | 15.5 (5.6) | 37.5 (7.0) | 9.52 (1.79) | 24 |
kip1Δ | 17.8 (4.8) | 35.1 (4.1) | 10.66 (1.35) | 24 |
bub2Δ kip1Δ | 5.4 (4.5) | 32.6 (3.7) | 9.89 (1.08) | 10 |
bim1Δ | 11.7 (6.2) | 28.1 (6.68) | 9.10 (1.16) | 21 |
All cells were filmed at 25°C as described in Materials and methods. All numbers reported are means (standard deviation). Time of MEN shows times after anaphase initiation (in minutes) of MEN (see Materials and methods). Final spindle lengths (µm) were measured from the final frame before breakdown. All strains that were compared to wild type (WT) and considered statistically significantly at P = 0.05 are shown in bold.