Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 11;12:14. doi: 10.1186/1471-2199-12-14

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2011 Koscianska et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Detection of heterogeneous miRNAs and pre-miRNAs. Examples of heterogeneous miRNAs and pre-miRNAs overexpressed in HEK 293T cells and detected with single-nucleotide resolution, as shown in the figure. A) miRNAs: miR-191, miR-496, miR-93 resolved in the range of ~17-25 nt. B) pre-miRNAs: pre-miR-191, pre-miR-496, and pre-miR-93 resolved in the range of ~60-70 nt. M-lanes denote the appropriate radiolabeled RNA oligonucleotide markers (ORNs); M1 denotes 17, 19, 21, 23, 25-nt ORNs for miRNA detection (A) and M2 denotes 60/61 and 63/64-nt ORNs for pre-miRNA detection (B). Quantitative representations of miRNA and pre-miRNA variants obtained from phosphorimaging analyses are shown schematically.