Table 2.
SYBR Green Real-time qPCR Primer sequences.
| Gene | Primer Sequence | GenBank AccessionNo. |
|---|---|---|
| human MMP-1 | Fw: 5'-AAG TTG AAA AGC GGA GAA ATA G-3' Rev: 5'-TTT CAA TCC TGT AGG TCA GAT G-3' |
NM 002421 |
| human MMP-2 | Fw: 5'-GGC AGA CAT CAT GAT CAA CT-3' Rev: 5' TGC TGT CAT AGG ATG TG-3' |
NG 008989 |
| human MMP-7 | Fw: 5'-AGT TTA GAA GCC AAA CTC AAG G-3' Rev: 5'-GCG GTA AGT CTC GAG TAT ATG-3' |
NM 002423 |
| human MMP-9 | Fw: 5'-TTG ACA GCG ACA AGA AGT GG-3' Rev: 5'-GTA CAT AGG GTA CAT GAG CG-3' |
NG 011468 |
| human MMP-13 | Fw: 5'-GCA GTC TTT CTT CGG CTT AG-3' Rev: 5'-GGA GTT ACA TCG GAC CAA AC-3' |
NM 002427 |
| human MMP-19 | Fw: 5'-CTT TCA AGG GGG ACT ATG TG-3' Rev: 5'-TAT TCA GCT TCT TGG GGA AG-3' |
NM 002429 |
| human MMP-28 | Fw: 5'-GAG GCA TTC CTA GAG AAG TAC G-3' Rev: 5'-CTA GCA AAC AAG TCA CTG ATC C-3' |
NM 024302 |
| human TNF-α | Fw: 5'-CAT GTT GTA GCA AAC CCT CA-3' Rev: 5'-CTT GGT CTG GTA GGA GAC G-3' |
NM 000594 |
| human 18sRNA | Fw: 5'-GAT CAG ATA CCG TCG TAG TTC C-3' Rev: 5'-TAT CAA TCT GTC AAT CCT GTC C-3' |
NR 003286 |