Table 1. Overexpression of GPAT1 in CHO cells increased intracellular levels of LPA, and lowered the content of acyl-CoA and DAG.

Lipid intermediates (nmol/g protein)	CHO	CHO-GPAT1
Acyl-CoA	566±317	38 *
Lysophosphatidic acid	236±38	1428±241 *
Diacylglycerol	7608±183	4452±1319 *

Lipid intermediates in CHO and CHO-GPAT1 cells were analyzed by mass spectrometry as described in Methods. Lysophosphatidic acid and diacylglycerol results are expressed as the mean ± SD; n = 3. The acyl-CoA result for CHO-GPAT1 is the average of 2 measurements (32 and 45); * p<0.01.