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. 2011 Apr 20;6(4):e18880. doi: 10.1371/journal.pone.0018880

Figure 5. Novel PD proteins identified through their subcellular targeting.

Figure 5

Transgenic expression of fluorescent fusion proteins and their targeting to puncta on the cell wall identified five new PD proteins. Panels A–F show projections of confocal laser scanning microscopy z-series of Arabidopsis leaf epidermal cells for YFP or mRFP fusions to the receptor-like kinases At1g56145(A, YFP), At4g21380(D, YFP), At5g24010 (E, RFP), and At5g59700 (F, YFP), hypothetical protein At3g15480 containing a DUF1218 domain (B, YFP) and a tetraspanin At3g45600 (C, YFP). PD localisation (arrowheads) is evident as punctae of fluorescence in the cell wall for images shown in A–E. For At1g56145 in (A), PD in pitfields at the epidermal-mesophyll boundary are visible (arrows). Panel F illustrates the targeting of a non-PD RLK At5g59700 to the PM. Bars  = 20 µm.