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. 2011 Apr 20;6(4):e18966. doi: 10.1371/journal.pone.0018966

Figure 5. Serum mitogens induce the positioning of a subset of the distal enhancers to the MYC promoter.

Figure 5

(A) HCT116 cells were grown to confluence, deprived of serum for 48 hrs and then stimulated with serum for 2 hours. Real-time PCR analysis was used to quantify MYC or Tubulin expression levels in cDNAs synthesized from RNAs isolated from starved (black bars) or serum-induced (grey bars). (B) Real-time PCR analysis of DNA fragments precipitated with either ß-catenin or TCF4 antibodies in ChIP assays conducted from HCT116 cells treated as in A. (C) Quantitative 3C analysis detected interactions between enhancers A through E and the MYC promoter in serum starved and serum activated HCT116 cells. In A, B and C data is normalized to levels measured in starved cells and error bars represent SEM.