FIG. 3.

TOP2A is inhibited by PARP1 and PARP2 activity. A) PARP1 inhibited TOP2A activity in the presence, but not in the absence, of NAD⁺ in an enzyme concentration-dependent manner. PARP1 itself exhibited no decatenating activity (lane 8). B) Similarly, PARP2 inhibited TOP2A activity in the presence, but not absence, of NAD⁺ in an enzyme concentration-dependent manner. C) TOP2A-dependent kDNA conversion rates from two independent experiments performed in duplicate are shown. Error bars indicate the standard deviations.