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S-nitrosylation of NOS3, HSP90, and β-actin in normotensive and preeclamptic placentas. The biotin-labeled SNO-proteins were further subjected to avidin capture. SNO-proteins, as well as total protein, were then separated by using 8% SDS-PAGE and immunoblotted with specific antibody. Four samples of each group were analyzed. Graph summarizes data (means ± SEM) and compares by t-test. * P < 0.05 vs. normal control.
