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. 2011 Apr 20;6(4):e19033. doi: 10.1371/journal.pone.0019033

Figure 6. Phenotypic analyses of reciprocal crosses between wild-type and amiR-atpv42b-1 plants.

Figure 6

(A) SEM of wild-type ovules at flower stage 12 prior to fertilization. (B, C) SEMs of developing seeds 2 days after pollination from the following reciprocal crosses: Col ♀×Col ♂ (B) and Col ♀× amiR-atpv42b-1 ♂ (C). (D) SEM of amiR-atpv42b-1 ovules at flower stage 12 prior to fertilization. (E, F) SEMs of developing seeds 2 days after pollination from the following reciprocal crosses: amiR-atpv42b-1 ♀×Col ♂ (E) and amiR-atpv42b-1 ♀× amiR-atpv42b-1 ♂ (F). Arrows indicate the unfertilized ovules. (G–J) Aniline blue staining of pollen tube growth inside pistils collected 20 hours after pollination from the following reciprocal crosses: Col ♀×Col ♂ (G), Col ♀×amiR-atpv42b-1 ♂ (H), amiR-atpv42b-1 ♀×Col ♂ (I), and amiR-atpv42b-1 ♀× amiR-atpv42b-1 ♂ (J). Arrows indicate the lateral growth of pollen tubes. Bars in (A-F), 100 µm.