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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Carcinog. 2010 Dec 10;50(5):346–352. doi: 10.1002/mc.20716

FIGURE 3. Fyn is necessary and sufficient for Ras-induced migration and invasion of HaCaT cells.

FIGURE 3

A, Fyn protein levels in HaCaT, HaCaT-Ras and HaCaT-Fyn cells were determined by western blotting (sc-434 antibody). Protein levels of α-Tubulin are shown as a loading control. B and C, The indicated cells were pretreated with CFDA fluorescence tracer and plated on FluoroBlok inserts without (B) or with (C) Matrigel coating. Migration and invasion were measured at 0, 24 and 48 hours, and all data was normalized to the zero time point to clearly show the percentage increase with time. T-test was performed on the indicated groups (*, #), p<0.01. D, HaCaT-Ras cells were transfected with either control or Fyn specific siRNA, and migration and invasion were measured after 24 and 48 hours, respectively. All data was normalized to the migration/invasion in control siRNA cells to clearly show the percentage decrease. Data is represented as mean ± SD from a representative experiment performed in triplicate. T-test was performed on the indicated groups (*, #), p<0.01.