Fig. 3.

Promoter activity assay for insulin and pancreatic and duodenal homeobox 1 (Pdx1) in INS-1 cells treated with 30 mM glucose and 0.25 mM palmitate. The promoter activities of insulin and Pdx1 were measured as described in Materials and Methods. RFU, rate fluorescence unit; LG, 5.6 mM glucose; HG, 30 mM glucose; Pal, 0.25 mM palmitate; NAC, N-acetyl-L-cysteine. ^aP < 0.05 vs. LG, ^bP < 0.05 vs. HG+Pal.