Fig. 6.
Impact of lipid conditions on LXA4 plasma levels and macrophage phagocytic activity. a Circulating LXA4 levels of apoE−/−/15-LOXtg+/0 (tg) and apoE−/− mice (+/+) fed a regular chow diet (n = 7 and 5) or a Western diet (WD, n = 4 and 4). ∗ p < 0.05, using Mann-Whitney rank sum test. b Plasma cytokine levels in transgenic mice (light gray bars) versus control (dark gray bars) under conditions of Western diet feeding. ∗ p < 0.05, ∗∗ p < 0.01, using Mann-Whitney rank sum test. c Macrophages (MPH) were treated with 50 nM of LXA4 (or vehicle) and co-incubated with fluorescent microspheres (for 70 min) in regular culture medium (RPMI with 10% FBS). High-lipid conditions are created by supplementing the media by additional 0.5 mg/dl of LDL to the phagocytosis medium; ∗∗ p < 0.01 (n = 4 for each set).