Fig. 4.

Mitochondrial permeability transition is inhibited by CK substrates. At a concentration of 10 mM, the CK substrates creatine (Cr) and cyclocreatine (cCr), Cr-analogon, inhibit MTP in isolated mouse liver mitochondria to a comparable degree as 1 μM cyclosporin A (CSA), the gold standard for MPT inhibition (Dolder et al. 2003). The Cr-analogon guanidinopropionic acid (GPA) that is not accepted as a substrate by the CK reaction has no effect as compared to control without additions (none). Isolated liver mitochondria from transgenic mice expressing uMtCK in their liver were analysed by a swelling (light scattering) assay. They were energized with glutamate/malate in presence of 2mM Mg²⁺ and then challenged by 120 μM Ca²⁺ where indicated. (Figure taken from Dolder et al. 2003 with permission)