Table 1.
Comparison of nestin-reporter mice.
| Yamaguchi et al. [8] | Aoki et al. [7] | Kawaguchi et al. [9] | Mignone et al. [12] | Beech et al. [11] | Our work | ||
|---|---|---|---|---|---|---|---|
| Transgenic strategy | Sequence that drives transgene | 2nd Intron, nestin promoter | 2nd Intron, thymidine kinase minimal promoter (a) | 2nd Intron, hsp68 min promoter | 2nd Intron, 5.8 kb nestin promoter | 5.8 kb upstream to 5.4 kb downstream of nestin Gene (b) | 2nd Intron, thymidine kinase promoter |
| Reporter molecule | eGFP | β-galactosidase | eGFP | eGFP | CREB | Modified (stable) eGFP (c) | |
|
| |||||||
| General features | Endogenous nestin coexpression | n.d | Yes | Yes (d) | Yes | n.d. (e) | Yes |
| Brdu+ | Yes (f) | n.d. (g) | Yes (h) | Yes | Yes | Yes | |
|
| |||||||
| Embryonic expression | Ventricular zone | Yes | Yes | Yes | Yes | n.d. | Yes |
| Subventricular zone | Yes | n.d. | Yes | Yes | n.d. | Yes | |
| Pallium versus subpallium | n.d. | n.d. | n.d. | No | n.d. | Yes | |
| 3d ventricle | Yes | Yes | Yes | Yes | n.d. | Yes | |
| Spinal cord | Yes | Yes | Yes | n.d. | n.d. | n.d. | |
| Postmitotic parencyma | Yes | n.d. | ? (i) | No | n.d. | Yes | |
| Nonneural tissues | No | No | n.d. | No | n.d. | Yes | |
|
| |||||||
| Postnasal and/or adult expression light microscopy | Neurogenic regions | Yes | Yes | Yes | Yes | Yes-subset (j) | Yes |
| Dentate gyrus progenitors | Yes | ? | n.d. | Yes | No | Yes | |
| Dentate gyrus neurons | Yes | ? | n.d. | No | No | No | |
| SVZ B cells | Yes | No | Yes (k) | Yes | ? (l) | Yes-subset | |
| SVZ C cells | n.d. | n.d. | No (m) | n.d. | ? | Yes-subset | |
| SVZ/RMS A cells | Yes | n.d. | n.d. | Yes | Yes-subset | Yes-subset | |
| Increased in SVZ after injury | n.d. | n.d. | n.d. | n.d. | n.d. | Yes | |
| Ependymal cells | Yes | n.d. | Yes | Yes | ? (l) | Yes-subset | |
| OB | |||||||
| Granule | Yes | ? | n.d. | No | No | Yes | |
| neurons | |||||||
| Periglomerular | Yes | ? | n.d. | Yes | Yes-subset | Yes | |
| neurons | |||||||
| Accessory OB | n.d. | n.d. | n.d. | n.d. | n.d. | Yes | |
| neurons | |||||||
| Nonneurogenic regions | Yes (n) | Yes | n.d. | No | Yes (o) | Yes | |
| Oligodendro- cytes | ? | n.d. | n.d. | No | n.d. | Yes | |
| Astrocytes | ? | No | n.d. | n.d. | n.d. | No | |
| Astrocytes after injury | n.d. | No (p) | n.d. | n.d. | n.d. | No | |
| Neurons | ? | Yes | n.d. | No | n.d. | Yes-weak | |
| Nonneural tissue | n.d. | n.d. | n.d. | n.d. | n.d. | Yes-hair follicles | |
|
| |||||||
| Electron microscopy | SVZ B cells | n.d. | n.d. | n.d. | n.d. | n.d. | Yes |
| SVZ C cells | n.d. | n.d. | n.d. | n.d. | n.d. | Yes | |
| SVZ A cells | n.d. | n.d. | n.d. | n.d. | n.d. | Yes | |
| Adult ependymal cells | n.d. | n.d. | n.d. | n.d. | n.d. | Yes | |
| Oligodendro- cytes | n.d. | n.d. | n.d. | n.d. | n.d. | Yes | |
|
| |||||||
| Cell culture | Neurospheres | n.d. | n.d. | Yes (q) | Yes | n.d. | Yes (r) |
n.d.: not determined. ?: not clear
(a) Line 3D6—includes mutation of SHP2 tyrosine phosphatase; (b) nestin-tTA mouse crossed with tetracycline regulated CREB used as readout; (c) Okada et al., 1999; (d) shown in vitro; (e) nestin expressed in the same general areas as transgene, cell coexpression not determined; (f) tested postnatally; (g) BrdU labeling index not changed, colabelling with transgene not done; (h) embryonically in vivo and in vitro; (i) parenchymal GFP+ cells visible in Figure 1(g); (j) SVZ but not dentate gyrus; (k) as determined by BrdU retention; (l) S 100b-labelling in Figure 4(a) looks ependymal not astrocytic; (m) determined by lack of BrdU pulse labelling; (n) glial morphology; (o) not labelled with BrdU; (p) not within 6 hrs post-MCAo; (q) embryonically, self-renewal and multipotency shown; (r) postnataly, self-renewal and multipotency shown.