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Forward and reverse primers used in quantitative real-time polymerase chain reaction
| Gene | Primer sequence (5’-3’) | Annealing temperature (°C) | Product size (bp) |
| β-actin | 5’-ACTGCCGCATCCTCTTCCTC-3’ 5’-ACTCCTGCTTGCTGATCCACAT-3’ | 55 | 598 |
| Rat TNF-α | 5’-GGCAGGTCTACTTTGGAGTCATTGC-3’ | 58 | 318 |
| 5’-ACATTCGGGGATCCAGTGAGCTCCG-3’ | |||
| Rat IL-6 | 5’-GGATACCACCCACAACAG-3’ 5’-GGTCCTTAGCCACTCCTT-3’ | 58 | 451 |
TNF: Tumor necrosis factor; IL: Interleukin.
