Fig. 4.
qRT-PCR analysis of HPV-positive SCCHN cell line SCC2 transfected with siRNA against HPV-16 E6. (A). qRT-PCR analysis of HPV-16 E6 oncogene expression in the HPV-positive cell line SCC2 upon transfection with a negative control siRNA or with siRNA against E6. (B). qRT-PCR analysis of miR-363 expression in cells transfected with a negative control siRNA or with siRNA against E6. No RT, no reverse transcriptase added. Intensity values are relative to the SCC2 cells transfected with a negative control siRNA, which were arbitrarily assigned a value of -1. The p-values for the SCC2 + siRNA E6 cells compared to the SCC2 with control siRNA are indicated by ** (p<0.01).