Table 2.
Plasmids used in this study.
| Plasmid | Description | Reference |
|---|---|---|
| pYL150 | 2μm, LEU2, Ampr, GAL10 promoter +CDC34 | [17] |
| pYL19 | pYL150 with S97D mutation in CDC34 | [17] |
| pYL27 | pYL150 with 12-residue deletion of G-103 to T-114 in CDC34 | [17] |
| pYL29 | pYL150 with 12-residue deletion of G-103 to T-114 and S73K and S97D mutations in CDC34 | [17] |
| pUBP14 | ADH1 promoter and terminator elements + UBP14 in pVT102-U | [38] |
| pUBP14-Ala354 | ADH1 promoter and terminator elements +catalytically inactive UBP14(C354A) mutant in pVT102-U | [38] |