Table 2.

Normal reference values for lymphocyte subsets in healthy adults determined by flow cytometry

Lymphocyte subset	95% reference values
	18-55 years	>55 years
CD45	0.99 - 3.15 thousand/uL	1.00 - 3.33 thousand/uL
CD3	677-2383 cells/μl	617-2254 cells/μl
CD4	424-1509 cells/μl	430-1513 cells/μl
CD8	169-955 cells/μl	101-839 cells/μl
CD19	99-527 cells/μl	31-409 cells/μl
CD16+56+	101-678 cells/μl	110-657 cells/μl
CD3	59-83%	49-87%
CD4	31-59%	32-67%
CD8	12-38%	8-40%
CD19	6-22%	3-20%
CD16+56+	6-27%	6-35%

Data derived from 207 healthy adult male and female donors. Pediatric reference ranges for T, B and NK cells [147].