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. 2011 Apr 21;7(4):e1001377. doi: 10.1371/journal.pgen.1001377

Figure 2. PDP-1 regulates multiple outputs of the IIS pathway.

Figure 2

Data shown are from one representative experiment. (A) pdp-1 RNAi does not significantly reduce the lifespan of wild-type worms. Mean lifespan of wild-type worms is 23.8±0.5 days (n = 93) on vector RNAi, 14.5±0.9 days (n = 34) on daf-18 RNAi (p<0.0001) and 22.6±0.6 days (n = 68) on pdp-1 RNAi (p<0.08). (B) The increased lifespan of daf-2(e1370) worms is reduced by pdp-1 RNAi. Mean lifespan of daf-2(e1370) worms is 38.9±0.9 days (n = 75) on vector RNAi, 24.5±0.5 days (n = 59) on daf-18 RNAi (p<0.0001) and 31.7±0.8 days (n = 66) on pdp-1 RNAi (p<0.0001). (C) pdp-1 RNAi reduces the increased lifespan of age-1(hx546) mutants. Mean lifespan of daf-2(e1370) worms is 42.8±0.8 days (n = 84) on vector RNAi, 28.0±0.9 days (n = 81) on daf-18 RNAi (p<0.0001) and 36.5±1.0 days (n = 67) on pdp-1 RNAi (p<0.0001). (D) pdp-1 overexpression increases the lifespan of wild-type and daf-2(e1370) worms while pdp-1 mutants live slightly shorter than wild-type animals. Mean lifespan of wild-type worms is 29.4±0.5 days (n = 104), pdp-1(tm3734) mutants was 27.1±0.7 days (n = 98), p<0.05, pdp-1::gfp mutants is 34.5±0.8 days (n = 92) p<0.0001, daf-2(e1370) is 38.7±0.7 days (n = 108) and daf-2(e1370); pdp-1::gfp is 42.8±0.7 days (n = 105) days p<0.0001. (E) PDP-1 regulates thermotolerance. Mean survival of wild-type worms is 18.3±0.7 hours (n = 37), pdp-1(tm3734) mutants is 17.1±0.8 hours (n = 27) p<0.2, pdp-1::gfp worms is 19.7±0.9 days (n = 25) p<0.09, daf-2(e1370) worms is 21.6±0.6 hours (n = 30) and pdp-1(tm3734); daf-2(e1370) worms is 18.6±0.9 hours (n = 19), p<0.0007). (F) Oil Red O staining reveals that pdp-1(tm3734); daf-2(e1370) worms store less fat than daf-2 worms across different stages in the worm life cycle: dauers (left), L3 worms (middle) and adults (right). Arrows indicate the lower bulb of the pharynx. (G) Quantification of Oil Red O staining in L3 and young adults of daf-2(e1370), pdp-1(tm3734); daf-2(e1370) and daf-2(e1370); pdp-1::gfp worms. A mutation in pdp-1 significantly reduces daf-2(e1370) fat storage in both, L3s (p<0.0001) and young adults (p<0.01). In adult worms, daf-2(e1370); pdp-1::gfp worms store slightly more fat than daf-2(e1370) not in younger L3 animals (p<0.02).