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. 2011 Apr 21;7(4):e1001370. doi: 10.1371/journal.pgen.1001370

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Skottke et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Wild-type and rcn1-6 mutant seedlings carrying Dexamethasone-inducible wild-type myc-ACS5 (A) or myc-ACS5^eto2 (B) transgenes were grown on media containing 25 nM Dexamethasone and extracts were harvested at time points after addition of the protein synthesis inhibitor cycloheximide. Immunoblots were probed with anti-myc to detect myc-ACS5 proteins and with anti-PEPC as a loading control.