Figure 6.

Nitric oxide [NO] donor dipropylenetriamine NONOate (DPTA-NO) treatment decreases brain hemorrhage incidence in Plasmodium berghei ANKA (PbA)-infected mice. Number of hemorrhages per section (A) and area occupied by hemorrhages in relation to the total area of the sections (B) in 4 regions of the brain in saline-treated (n = 9) and DPTA-NO–treated (n = 10) PbA-infected mice. After formalin fixation, the brain was cut (coronal plane) in 4 slices of 2–3mm each, and each region was defined as frontal lobe/olfactory bulb (FL); midbrain anterior (MA); midbrain posterior (MP); or cerebellum-brainstem (Ce). Five sections that were 400 μm apart were examined per region, with a total of 20 sections analyzed per mouse. #: P < .05 compared with DPTA-NO. Statistical analysis was performed by 1-way analysis of variance (ANOVA) for nonparametric repeated measurements, and post hoc analyses were performed using Bonferroni post tests. Data are from 2 experiments.