Table 2.
Virus Titers in Primary DENV Infection Determined Using BHK and BHK- FcγRIIA Cell Lines
| Serum | DENV | Log10 genome | Virus titers (log10 PFU/mL) |
||||
| ID no. | Type | Daysa | equivalents/mL | BHK (VO) | FcγRIIA (VN) | Ratio (VN/VO) | P-value (VN,VO) |
| Anti-DENV IgM -, IgG - | |||||||
| P1ac | DENV-1 | 0 | 6.56 | 6.45 ± 0.10 | 6.49 ± 0.03 | 1.1 | .227 |
| P2a | DENV-1 | 1 | 8.60 | 7.32 ± 0.03 | 7.34 ± 0.10 | 1.0 | .449 |
| P3a | DENV-1 | 1 | 7.04 | 6.59 ± 0.01 | 6.50 ± 0.07 | 1.2 | .070 |
| P4a | DENV-1 | 1 | 7.38 | 5.41 ± 0.02 | 5.36 ± 0.15 | 1.0 | .488 |
| P5 | DENV-1 | 1 | 6.27 | 3.95 ± 0.21 | 4.00 ± 0.19 | 1.1 | .364 |
| P6a | DENV-2 | 1 | 7.63 | 6.90 ± 0.33 | 6.90 ± 0.22 | 1.0 | .458 |
| P7 | DENV-2 | 1 | 7.48 | 5.32 ± 0.03 | 5.35 ± 0.09 | 1.0 | .388 |
| P8 | DENV-3 | 1 | 9.38 | 5.93 ± 0.04 | 6.03 ± 0.08 | 1.3 | .105 |
| P9 | DENV-3 | 1 | 8.92 | 6.74 ± 0.01 | 6.72 ± 0.06 | 0.9 | .320 |
| P10a | DENV-1 | 2 | 7.48 | 6.66 ± 0.08 | 6.80 ± 0.04 | 1.3 | .040 |
| P11a | DENV-1 | 2 | 7.93 | 6.10 ± 0.02 | 6.22 ± 0.09 | 1.1 | .241 |
| P12a | DENV-3 | 2 | 8.49 | 6.70 ± 0.12 | 6.90 ± 0.04 | 1.5 | .078 |
| P13 | DENV-4 | 2 | 8.76 | 5.45 ± 0.01 | 5.38 ± 0.05 | 0.8 | .060 |
| P14 | DENV-2 | 3 | 6.75 | 5.15 ± 0.09 | 5.10 ± 0.13 | 0.8 | .303 |
| P15 | DENV-2 | 4 | 4.81 | 3.08 ± 0.21 | 3.04 ± 0.13 | 0.9 | .425 |
| P6b | DENV-2 | 5 | 6.36 | <2.30 | <2.30 | -b | -b |
| Anti-DENV IgM +, IgG - | |||||||
| P16 | DENV-3 | 1 | 7.76 | 6.74 ± 0.01 | 6.72 ± 0.06 | 1.2 | .332 |
| P17 | DENV-3 | 1 | 5.55 | <2.30 | <2.30 | - | - |
| P18 | DENV-1 | 2 | 6.00 | 4.15 ± 0.28 | 4.09 ± 0.14 | 0.8 | .447 |
| P19 | DENV-2 | 3 | 5.67 | 4.38 ± 0.05 | 4.39 ± 0.15 | 1.0 | .473 |
| P20 | DENV-3 | 3 | 9.20 | 6.61 ± 0.31 | 6.57 ± 0.14 | 0.9 | .494 |
| P12b | DENV-3 | 3 | 7.82 | 5.19 ± 0.02 | 5.16 ± 0.04 | 0.9 | .330 |
| P21 | DENV-3 | 3 | 6.68 | <2.30 | <2.30 | - | - |
| P22 | DENV-4 | 3 | 7.51 | <2.30 | <2.30 | - | - |
| P3b | DENV-1 | 4 | 4.64 | <2.30 | <2.30 | - | - |
| P23 | DENV-2 | 4 | 6.15 | 4.41 ± 0.05 | 4.38 ± 0.10 | 0.9 | .311 |
| P24 | DENV-3 | 4 | 8.46 | 5.11 ± 0.09 | 5.11 ± 0.07 | 1.0 | .458 |
| P25 | DENV-4 | 4 | 8.23 | 3.88 ± 0.04 | 3.98 ± 0.18 | 1.2 | .311 |
| P26 | DENV-1 | 5 | 6.00 | 4.13 ± 0.05 | 4.19 ± 0.05 | 1.1 | .277 |
| P27 | DENV-1 | 5 | 5.73 | <2.30 | <2.30 | - | - |
| P28 | DENV-3 | 5 | 6.28 | 3.30 ± 0.19 | 3.19 ± 0.23 | 0.7 | .259 |
| P29 | DENV-3 | 5 | 7.08 | <2.30 | <2.30 | - | - |
| P30 | DENV-4 | 5 | 6.97 | <2.30 | <2.30 | - | - |
| Anti-DENV IgM +, IgG+ | |||||||
| P1b | DENV-1 | 5 | - | <2.30 | <2.30 | - | - |
| P11b | DENV-1 | 5 | - | <2.30 | <2.30 | - | - |
| P2b | DENV-1 | 5 | - | <2.30 | <2.30 | - | - |
| P10b | DENV-1 | 6 | - | <2.30 | <2.30 | - | - |
| P31d | DENV-3 | 6 | - | <2.30 | <2.30 | - | - |
| P32d | DENV-3 | 6 | 5.48 | <2.30 | <2.30 | - | - |
| P33d | DENV-3 | 6 | - | <2.30 | <2.30 | - | - |
| P4b | DENV-1 | 7 | - | <2.30 | <2.30 | - | - |
| P10c | DENV-1 | 7 | - | <2.30 | <2.30 | - | - |
NOTE. a Days indicates days after onset of disease.
b
– indicates that calculation is not possible or below detection limits.
c
“a”, “b” and “c” indicates serial serum samples collected from a single patient
d
Patients P31, P32, and P33 were confirmed anti-DENV IgG negative during the early phase of the disease or 1–3 days after onset of the disease.