Table 3.
Virus Titers in Secondary DENV Infection Determined Using BHK and BHK- FcγRIIA Cell Lines
| Serum | DENV | Log 10 Genome | Virus titers (log10 PFU/mL) |
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| ID no. | Type | Daysa | equivalents/mL | BHK (VO) | FcγRIIA (VN) | Ratio (VN/VO) | P-value(VN,VO) |
| Anti-DENV IgM -, IgG+ | |||||||
| S1ac | DENV-2 | 1 | 8.40 | 7.00 ± 0.12 | 8.17 ± 0.15 | 14.0 | .002 |
| S2a | DENV-2 | 1 | 8.57 | 6.95 ± 0.38 | 8.56 ± 0.07 | 40.0 | .049 |
| S3a | DENV-2 | 1 | 9.11 | 6.81 ± 0.24 | 7.51 ± 0.14 | 4.9 | .021 |
| S4 | DENV-3 | 1 | 7.50 | 7.06 ± 0.19 | 8.26 ± 0.12 | 16.4 | .019 |
| S5a | DENV-2 | 2 | 7.83 | 6.29 ± 0.04 | 6.76 ± 0.07 | 3.0 | .001 |
| S6a | DENV-3 | 2 | 7.88 | <2.30 | <2.30 | -b | -b |
| S7 | DENV-4 | 2 | 8.45 | 5.18 ± 0.21 | 5.27 ± 0.13 | 1.2 | .306 |
| S8 | DENV-1 | 3 | 6.83 | 5.76 ± 0.03 | 6.77 ± 0.07 | 10.3 | <.001 |
| S9 | DENV-2 | 3 | 9.30 | 6.59 ± 0.14 | 7.71 ± 0.05 | 10.0 | .019 |
| S2b | DENV-2 | 3 | 8.04 | 6.53 ± 0.07 | 8.01 ± 0.04 | 38.2 | .004 |
| S10 | DENV-4 | 3 | 7.87 | 3.49 ± 0.02 | 4.70 ± 0.22 | 16.1 | <.001 |
| S11 | DENV-1 | 4 | 6.28 | 5.11 ± 0.37 | 5.92 ± 0.12 | 4.1 | .004 |
| S12a | DENV-1 | 4 | 7.66 | <2.30 | <2.30 | - | - |
| S13 | DENV-1 | 5 | 6.55 | 5.04 ± 0.05 | 5.93 ± 0.15 | 7.7 | <.001 |
| S14 | DENV-3 | 5 | 5.99 | 4.20 ± 0.15 | 4.26 ± 0.07 | 1.1 | .341 |
| Anti-DENV IgM +, IgG+ | |||||||
| S15a | DENV-4 | 1 | 8.76 | 6.08 ± 0.21 | 7.53 ± 0.06 | 27.5 | .028 |
| S1b | DENV-2 | 3 | 8.75 | 5.77 ± 0.01 | 6.70 ± 0.10 | 8.3 | <.001 |
| S5b | DENV-2 | 3 | 6.20 | 5.11 ± 0.04 | 6.10 ± 0.06 | 9.2 | <.001 |
| S16 | DENV-3 | 3 | 8.11 | <2.30 | 4.85 ± 0.33 | >3500 | .021 |
| S17 | DENV-1 | 4 | 5.18 | <2.30 | <2.30 | - | - |
| S2c | DENV-2 | 4 | 7.23 | 6.15 ± 0.04 | 7.18 ± 0.11 | 10.7 | <.001 |
| S3b | DENV-2 | 4 | 4.00 | <2.30 | <2.30 | - | - |
| S18 | DENV-3 | 4 | 6.68 | 3.20 ± 0.24 | 4.10 ± 0.15 | 7.5 | .037 |
| S19 | DENV-4 | 4 | 8.76 | 5.15 ± 0.09 | 6.24 ± 0.18 | 12.1 | <.001 |
| S15b | DENV-4 | 4 | 7.67 | 3.60 ± 0.34 | 4.65 ± 0.25 | 11.2 | .042 |
| S1c | DENV-2 | 5 | 4.30 | <2.30 | <2.30 | - | - |
| S12b | DENV-1 | 6 | - | <2.30 | <2.30 | - | - |
| S3c | DENV-2 | 6 | 3.74 | <2.30 | <2.30 | - | - |
| S20a | DENV-3 | 6 | 7.48 | 2.95 ± 0.07 | 4.26 ± 0.05 | 20.0 | .002 |
| S21 | DENV-3 | 6 | - | <2.30 | <2.30 | - | - |
| S20b | DENV-3 | 7 | 5.46 | <2.30 | <2.30 | - | - |
NOTE. a Days indicates days after onset of disease.
b
– indicates that calculation is not possible or below detection limits.
c
“a”, “b” and “c” indicates serial serum samples collected from a single patient.