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. 2011 Apr 21;6(4):e19090. doi: 10.1371/journal.pone.0019090

Figure 3. Dorso-ventral (D–V) and antero-posterior (A–P) patterning is normal in Atf2 − /− brainstem at E10.5.

Figure 3

(A) Double immunofluorescence staining for class III β-tubulin (Tuj1, red) and for neuronal class progenitor cells markers Nkx2.2, Pax6, Pax7 (all green) and postmitotic marker Isl-1 (green) in Atf2−/− and Atf2+/− brainstem. No significant difference was observed in the expression domains of the transcription factors between the two genotypes. Bar: 100 µm. (B) Whole-mount in situ hybridization for Krox20 and Hoxb3. No differences were observed in the expression domain between the two genotypes. r4, r5, rhombomere 4 and 5. Note that, at this stage, Krox20 expression is seen in rhombomere 5 (r5) only. (C) Atf2−/− and Atf2+/− whole-mount immunostaining with NF-M antibody at E12.5. Fiber nerves extend normally from Atf2−/− hypoglossal (XII) and vagal (X) nuclei. C1, C2, first and second cervical nerve, XII, hypoglossal nerve, X, IX, vagal and glossopharyngeal nerves, VII, VIII, acousticofacial nerve, V3, mandibular nerve, V2, maxillary nerve, V1, ophthalmic nerve.