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. 2011 Apr 21;6(4):e19090. doi: 10.1371/journal.pone.0019090

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Ackermann et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A–D) Immunostaining against NF-M (A and C) and phosphorylated NF-H (B and D) at E12.5 reveals strong accumulation of NF-M in the soma of Atf2^−/− hypoglossal motoneurons (h) but not in Atf2^+/− motoneurons (compare A and C). Phosphorylation of NF-H as detected with the antibody RMO24.9 was not yet observed at this age. (E–H) Immunostaining against NF-M (E and G) and phosphorylated NF-H (F and H) at E14.5 reveals strong accumulation of NF-M in hypoglossal (h) and vagal (v) motoneurons in Atf2^−/− (E) compared to Atf2^+/− embryos (G). Phosphorylation of NF-H can be detected in Atf2^−/− motoneurons (F, arrowheads) but not in Atf2^+/− neurons (H). Inset: magnification of P-NF-H stained hypoglossal neurons. (I-L) Immunostaining against NF-M (I and K) and phosphorylated NF-H (J and L) at E15.5 reveals aberrant accumulation of NF-M and hyperphosphorylation of NF-H in the soma of Atf2^−/− spinal cord motoneurons at the C1 level (C1 mn) but not in Atf2^+/− neurons. Bar: 100 µm. Inset: magnification of P-NF-H stained C1 motoneurons. Bar: 25 µm.