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. 2011 Apr 21;6(4):e19250. doi: 10.1371/journal.pone.0019250

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Hamazaki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Step1: KAN-MX6 cassette at the AAC2 locus in AAC triple mutant yeast (TCY119) was replaced with URA3 to establish the parent stain (URA-AAC2). Step2: PCR-generated N-terminal AAC + hANTs ORF fragments (yNhANTs) were used for transformation of URA-AAC2, and transformants were selected on rich glucose media containing 5-FOA.