Figure 3. Over-production of yNhANT4 by high-copy plasmid inhibits yeast cell growth.

Both yNhANT4 and yNhANT2 ORF were cloned into a high-copy plasmid and expressed under the control of the GAL1 promoter. Three independent isolates, each transformed with one of the indicated plasmids (Empty plasmid, yNhANT4, and yNhANT2) were streaked on medium as indicated. (A) glucose medium, (B) galactose medium, and (C) YPEG + 0.1% galactose.