Figure 3.

Inhibition of PG synthesis in fetal testes from GD14.5 rats (A–E) and SC5 juvenile mouse Sertoli cells (F). (A) MBP (10 μM) inhibits secretion of PGD2 after 24 hr culture. (B) MEHP (10 μM) weakly inhibits PGD2 secretion after 24 hr. (C) The inhibitory action of MEHP is evident after stimulation of PGD2 synthesis with 100 μM AA for all time points, also implying that the inhibition is downstream from AA. The CYP17 inhibitor ketoconazole reduced testosterone production (D) but did not affect PGD2 synthesis (E). (F) Stimulation of PGD2 secretion from SC5 cells with 1 and 100 μM AA is inhibited by DBP, BPa, BP3, and BPA, normalized (norm) to control values. Similar action is seen with pharmaceutical inhibitors ASA, Ace, and Ibu, indicating that DBP, BPa, BP3, and BPA are inhibiting the COX enzymes. Data are mean ± SE for three experiments performed in triplicate.

*p < 0.05, **p < 0.01, and ^#p < 0.001, compared with controls by two-tailed Student’s t-test.