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. 2011 Apr 5;108(16):6549–6554. doi: 10.1073/pnas.1018981108

Fig. 2.

Fig. 2.

LPPs capture performance related to GC content and length. (A) The box plots show the distribution in GC content (y axis) of five groups of exons, defined on the basis of amplification success (R ≥ 0.9) in 63 DNA samples. These groups contain exons that amplified in all samples (group 1: 4,427 exons), exons that failed in only a subset of samples including in 1–10 samples (group 2: 522 exons), 11–40 samples (group 3: 284 exons), 41–62 samples (group 4: 242 exons), and exons that failed in all samples (group 5: 144 exons). Group 1 with the highest amplification success had the lowest mean GC content compared with groups 2–5 (P < 0.0001). (B) The box plots show the fraction of failed samples in four amplicon groups that we defined on the basis of amplicon length and GC content. Amplicons with lower GC content (<37%; <20th percentile) amplified successfully irrespective of their length, whereas amplicons with higher GC content (>61%; >80th percentile) had a tendency for amplification failures and in particular in longer amplicons (>274 bp; >80th percentile).