Table 1.
Step | Total protein (mg) | Total activity (units) | Specific activity (units/mg) | Yield (%) |
Cell-free extract | 3,050 | 4.88 | 0.0016 | 100 |
DEAE Sepharose | 292 | 4.99 | 0.0171 | 102 |
Blue Sepharose | 8.80 | 0.299 | 0.0340 | 6.13 |
HiTrap Benzamidine | 0.52 | 0.229 | 0.440 | 4.69 |
PIKSI Red 3 A6XL | 0.04 | 0.070 | 1.76 | 1.44 |