Skip to main content
. 2011 Apr 1;108(16):6597–6602. doi: 10.1073/pnas.1007694108

Fig. 3.

Fig. 3.

Knockdown of NUAK2 in C32 melanoma cells results in reduced proliferation and migration. (A) Cell number analysis of C32 melanoma cells with knockdown of NUAK2. Knockdown of NUAK2 dramatically reduced cell proliferation. (B) SA-β-gal staining in C32 melanoma cells. Images obtained using phase-contrast (Upper) and bright-field (Lower) microscopy are shown. Staining indicates senescent cells. Percentages of positive cells and SE values are given below the images. shEV, empty vector. (C) Cell-cycle profiles of C32 melanoma cells with knockdown of NUAK2. A significant decrease of the S-phase population in cell-cycle profiles is observed. (D) Wound-healing assay using C32 melanoma cells with knockdown of NUAK2. Knockdown of NUAK2 significantly decreased the migration of C32 melanoma cells. (E) Knockdown of NUAK2 by shNUAK2 significantly decreased the expression of mTOR. (F) Tumor growth of C32 melanoma cells infected with shNUAK2 relative to the empty vector. Tumor growth was significantly suppressed by infection of shNUAK2.