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. 2011 Apr 4;108(16):6543–6548. doi: 10.1073/pnas.1103471108

Fig. 4.

Fig. 4.

Effects on growth in yeast of mutations in HTS1 analogous to mutations in HARS2. HTS1-L198V, corresponding to HARS2 p.L200V, complements loss of HTS1 more effectively than does HTS1-V381L, corresponding to HARS2 p.V368L. HTS1-Δ198–205, corresponding to HARS2 p.Δ200–211, fails to complement loss of HTS1. (A) hts1Δ yeast with a rescuing wild-type copy of HTS1 on a URA3 plasmid were transformed with TRP1 plasmids encoding HTS1 of various genotypes. The resulting strains were streaked on Trp− media containing 5-fluoroorotic acid, to select against the rescuing wild-type HTS1. Growth indicates complementation by wild-type or mutant HTS1 on the TRP plasmid. (B) Growth in liquid culture of the strains from A.