Fig. 3.

Reg1 is required for Snf1 dephosphorylation in response to glucose replenishment. Strains were as in Fig. 2. (A) Immunoblot analysis was as in Fig. 1B except that, after cells were resuspended in SC plus 0.05% glucose for 10 min, an aliquot was incubated in SC plus 2% glucose (+G) for 15 min and collected. Values indicate relative intensity of the bands corresponding to phosphorylated Snf1-Thr210 and total Snf1 protein (pT210/Snf1). (B) Cells were grown to midlog phase in SC plus 2% glycerol and 3% ethanol (GE); an aliquot was shifted to SC plus 2% glucose for 15 min (G). Cells were collected and analyzed as above.