Fig. 5.

Regulation of Snf1 Thr210 phosphorylation in the presence of different β-subunits. Cells were grown in SC plus 2% glucose (H) or shifted to 0.05% glucose (L) and collected for immunoblot analysis of phosphorylated Thr210 and Snf1. (A) Strains were derivatives of W303 carrying REG1 (WT) or reg1Δ (Δ). (B) Gal83 (WT) and its derivative Gal83W184A were expressed as fusions to GFP from the native promoter on a centromeric plasmid (22) in gal83Δ sip1Δ sip2Δ (βΔ) or reg1Δ gal83Δ sip1Δ sip2Δ (reg1Δ βΔ) cells. All lanes are from the same blot.