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Conformational distribution of the early intermediate is broad and is restricted by formation of the stable complex or the cargo. (Left) Positions of the G–G (A), NG–NG (B), and N–N (C) FRET pairs in the stable SRP•SR NG-domain complex. (Right) FRET distance distributions, P(r), for each FRET pair in the early intermediate (blue), stable complex (red), and the early intermediate bound to cargo (green), derived from maximal entropy analyses of the TR-FRET data as described in SI Methods.
