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Effect of balanced amino acid feeding strategy in fed-batch culture of CHO-C12 cells. Parallel fed-batch cultures were carried out using the feed medium with imbalanced (closed icons) and balanced amino acid concentrations (open icons). a,b: amino acid concentrations; c, d: viable cell densities and Ab concentrations; e,f: glucose and lactate concentrations; g, h: glutamine, ammonia and alanine concentrations
